Figure 1.

Western blot analysis of the effect of epinephrine on the protein level of angiopoietin-like protein 2 (ANGPTL2) in differentiated 3T3-L1 adipocytes. (A) Differentiated 3T3-L1 cells were treated with epinephrine in different concentrations (E10, 30, or 50 nM) for 24 h in the presence or absence of phentolamine (Phe, 10 μM), propranolol (Pro, 0.3 μM), LY294002 (50 μM) or protein kinase A inhibitor fragment 6–22 amide (PKAI, 1 mM). Cell lysates were subject to western blot analyses for ANGPTL2 expression. Lysates from untreated cells were used as a control. β-Actin blotting was used as a loading control. (B) ANGPTL2 and β-actin blots were measured by densitometry. The density of the ANGPTL2 blot was normalized against that of β-actin to obtain a relative density, which was expressed as fold change to the relative blot density of the control (designated as 1). ^aP<0.05 compared with untreated control cells; ^bP<0.05 compared with E10 nM; ^cP<0.05 compared with E30 nM; ^dP<0.05 compared with E50; ^eP<0.05 compared with E50 nM+Phe (10 μM).