Figure 5.
Combination of FOXM1/proteasome inhibitors and ROS inducer PEITC synergistically inhibits cell viability and long-term colony formation. A: Combination of PEITC with bortezomib (bor) or thiostrepton (thio), respectively, is synergistic in inhibiting proliferation of cancer cells. The CI chart for the PEITC/bor or PEITC/thio drug combinations was plotted with CI on the y axis and fractional effect on the x axis. Percentage inhibition of viability was determined after treatment with a single agent or the PEITC/bor and PEITC/thio combination, respectively. The CI values of the agents are plotted for MIA PaCa-2 pancreatic and MDA-MB-231 breast cancer cells. B: A total of 1 × 105 MIA PaCa-2 human pancreatic cancer cells were plated and treated as indicated for 24 hours. Ten days after treatment, cells were stained with crystal violet and representative plates are shown. C: Graph shows the quantification means ± SD of duplicate experiments. D: A total of 1 × 105 MDA-MB-231 human breast cancer cells were plated and treated as indicated for 24 hours. Ten days after treatment, cells were stained with crystal violet and representative plates are shown. E: Graph shows the quantification means ± SD of duplicate experiments.