Figure 3.

Collection of fornicial epithelial samples by LCM. Sections of conjunctival fornix cul-de-sac from 8-week-old Spdef^+/+ mice and Spdef^−/− mice were collected then stained with H&E, and 40% of the fornicial epithelium, as measured from the deepest point of the fornix cul-de-sac, was collected for RNA isolation and microarray analysis. Conjunctival epithelium from one eye of one male and one female animal were pooled into a single sample. Images show fornicial tissue before (left panels; boxed areas) and after (right panels) laser microdissection. Scale bar = 200 μm.