Figure 5.

The secreted Wnt inhibitor Frzb is specific to the goblet cells in the conjunctival epithelium. A: Multiple clusters of goblet cells (top panels) or areas of stratified epithelium without goblet cells (bottom panels) from Spdef^+/+ mice were collected, separately, by LCM. Images show tissue stained with H&E before and after laser microdissection. B: Frzb mRNA was detected only in goblet cell samples and not in the stratified epithelial samples. Error bars represent means ± SEM. ^∗∗P < 0.01. C: Frzb protein (green) was localized by immunofluorescence to goblet cells within the conjunctival epithelium (arrows; left panel). Phase contrast image (middle panel) allows for better visualization of epithelial and goblet cell (arrows) morphology, and the merged immunofluorescent and phase image (right panel) demonstrates localization of Frzb to goblet cells within the conjunctival epithelium (arrows). Scale bars: = 100 μm (A); 50 μm (B).