Figure 1.

Increased dilated membranous organelles and higher cytoplasmic immunoreactivity for mutant decorin in 952delTDcn keratocytes. A: Decorin localized almost exclusively in the stromal extracellular matrix in WT corneas. B: Virtually no immunoreactivity of decorin was detected in the corneal stroma from decorin-deficient mice. C: Immunolocalization of the mutant decorin revealed higher reactivity of mutant decorin (white arrows) in the keratocyte cytoplasm than in the extracellular matrix in a mutant mouse with decorin-null background. Exposures in mice with decorin-null background were twofold those in WT mice. Yellow arrows indicate the corneal stroma. D: Ultrastructural analysis of the corneal stroma showed flat keratocytes along the lamellar layer in WT mice. E: In contrast, keratocytes were substantially enlarged, with increased dilated membranous organelles (arrows), in 952delTDcn mice.