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. 2013 Jul;183(1):247–256. doi: 10.1016/j.ajpath.2013.04.001

Figure 5.

Figure 5

Mutant decorin is retained in the ER with abnormal transport/secretion. A–C: HEK293T cells were seeded on collagen I–coated coverslips and transfected with either WT or 952delT decorin cDNA. Immunocolocalization demonstrated low immunoreactivity of decorin in the ER and higher scattered punctate immunoreactivity that did not colocalize with the ER marker (arrow in B) in cells transfected with WT decorin. D–F: Substantially higher decorin immunoreactivity was colocalized with the ER (arrows) in cells transfected with 952delT decorin. G–I: Immunocolocalization indicated that punctate WT decorin colocalized with the Golgi marker (arrows). J–L: In HEK293T cells transfected with mutant decorin, although mutant decorin was highly expressed in the cytoplasm compared with the controls, it did not colocalize with the Golgi marker. There was limited reactivity against mutant decorin that colocalized with the Golgi marker (yellow pixels indicated by the arrowhead in L). A part of the Golgi is not colocalized with decorin (green pixels indicated by the arrow in L). Scale bars: 25 μm (all panels).