Figure 6.

Mutant decorin is degraded through the ubiquitin-proteasome pathway. HEK293T cells were transfected with either WT or mutant decorin cDNA. At 36 hours after transfection, the cells were treated with cycloheximide without (A and B) or with (C and D) MG132. Cells were trypsinized, washed with PBS, and collected at 0, 6, 12, and 24 hours after treatment. A and B: Immunoblots showed that intracellular decorin levels decreased over time after treatment with cycloheximide. C and D: Combined treatment with cycloheximide and MG132 substantially increased intracellular decorin levels in cells transfected with 952delT decorin when compared with those treated with WT decorin.