Figure 7.

Retention of mutant decorin in the ER leads to ER stress. Immunoblots demonstrate substantially increased expression of CHOP, BiP, and PERK in HEK293T cells transfected with mutant decorin compared with those with WT decorin. Anti-actin bands were used as loading controls, and anti–green fluorescent protein bands as transfection controls. Transfected HEK293 cells were collected, lyzed, and loaded onto Phos-tag gels. Anti–phospho-PERK immunoblots show substantially increased reactivity in cells transfected with 952delT decorin compared with those transfected with WT decorin.