Figure 2.

Mildest visible lesion in arterioles supplying the distal femoral epiphysis in steroid-treated mice. A: H&E staining shows segmental mild thickening of vessel wall, increased basophilia, and loss of smooth muscle cell and endothelial cell nuclei in area indicated by arrow. B: Masson’s trichrome, segmental loss of trichrome-positive staining in area of vascular smooth muscle cells. Segment indicated by arrow. C: α-SMA immunohistochemistry shows loss of α-SMA in area of smooth muscle necrosis. Segment indicated by arrow. D: CD31 immunohistochemistry shows segmental loss of endothelial cells. Area indicated by the arrow. Original magnification: ×600 (A–D).