Figure 3.

Vascular lesions in arterioles supplying the distal femoral epiphysis. A: H&E staining of cross-section of arteriole shows transmural necrosis and the absence of intraluminal obstruction. B: H&E staining of cross-section of arteriole shows transmural necrosis, thickening of the vessel wall, and partial luminal obstruction. C: H&E staining shows diffuse transmural necrosis with segmental thickening of the vessel wall and partial loss of the internal elastic lamina. D: Elastin histochemistry performed after removal of coverslip and de-staining of section in C, showing segmental loss and discontinuity of the internal elastic lamina, indicated by arrow. E: H&E staining of sagittal section of distal femoral epiphysis shows complete occlusion of vascular lumen by a thrombus proximal to the area of mural thickening (boxed area) and diffuse, extensive bone marrow necrosis (asterisk) and focal, partial necrosis of trabecular bone. F: Magnification of boxed area in E. G: H&E staining of sagittal section of distal femoral epiphysis, physis, and metaphysis shows thrombosis of segment of vessel located proximal to the vessel shown in boxed area of E, concurrent with diffuse, extensive bone marrow and trabecular bone necrosis (asterisk). H: Magnification of boxed area in G. Original magnification: ×600 (A); ×400 (B–D, F, and H); ×100 (E and G).