Table 3.

Liver Regeneration, Serum ALT, and Hepatic Hydroxyproline Levels in Fibrotic Mice Treated with TWEAK and Anti-TWEAK after 70% Hepatectomy

Group	Regeneration ratio (%)	ALT (U/L)	Collagen (ug/100 mg)	Proliferation (Ki-67+ cells/high-power fields)
				Hepatocytes	Ductular cells
P1.71	49.69 ± 4.54	306 ± 88	41.14 ± 3.05	9.66 ± 0.87	10.33 ± 1.24
TWEAK	50.80 ± 4.74	153.6 ± 60.6	44.50 ± 2.85	9.15 ± 1.21	24.47 ± 2.88∗
Anti-TWEAK	62.89 ± 2.58∗	48.5 ± 23.3∗	33.95 ± 2.35	17.76 ± 1.14∗	3.80 ± 0.29∗

Regeneration ratio was assessed by calculating each regenerated liver weight at the time of sacrifice (day 5) as percentage of pre-resection liver weight (estimated based on weight of resected lobes). Serum alanine aminotransferase (ALT) levels and hepatic collagen (as hydroxyproline content) were measured at sacrifice, as described in Materials and Methods (n = 6 to 10 mice per group). Cell proliferation was assessed by quantification of Ki-67⁺ hepatocytes and Ki-67⁺ ductular cells (see staining in Figure 5, C and D). Ten random high-power fields at ×200 magnification were counted in 4 to 5 mice per group. Data are expressed as means ± SEM.

^∗P < 0.05 versus P1.71 control group.