Skip to main content
. 2013 Jul;183(1):296–303. doi: 10.1016/j.ajpath.2013.03.020

Figure 1.

Figure 1

Natural and induced multinuclearity is induced by FAM190A absence. A: H&E-stained section of a pancreatic cancer tissue with known FAM190A exon 4 to 5 deletions. The tissue has focal multinuclear cells (arrow). B: HeLa cell lines were generated by stable transfection of control shRNA plasmid or four different FAM190A-targeting shRNA plasmids. FAM190A-targeting shRNA clone 77-2 had a reduced level of FAM190A protein compared with control shRNA clone 03-1. IB with mAb7 and anti–α-tubulin. C: Agarose plugs were prepared from two shRNA lines from B. Multinuclear cells were present in clone 77-2 by H&E staining (arrow). Control cells lacked multinuclearity. D: Phase-contrast images of FAM190A-knockdown clone 77-2 and control cells. Multinuclear cells were present in clone 77-2. E: Phase-contrast images from a time-lapse movie of FAM190A-knockdown clone 77-2 (Supplemental Video S1). A multinuclear cell underwent multiple rounds of cell division, generating progressively more nuclei in the cell (arrow).