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. 2013 Jul;183(1):296–303. doi: 10.1016/j.ajpath.2013.03.020

Figure 2.

Figure 2

FAM190A cellular localization suggests mitotic functions. A: Representative confocal IF images of HeLa cells at various cell cycle stages. Red, FAM190A; green, α-tubulin; blue, DNA. B: Representative IF image of Flemming body of HeLa cells. Red, FAM190A; green, α-tubulin. C: Representative IF image of midbody of FAM190A-knockdown clone 77-2 and control cells. Red, FAM190A; green, α-tubulin. D: Representative images from IHC of HeLa cells using anti-FAM190A antibody (mAb7), cells in mitosis. E: IB comparing nuclear and cytoplasmic fractions of HeLa cells. IB with mAb7, anti–α-tubulin, and anti–Lamin B. C, cytoplasmic; N, nuclear; T, total. Scale bars: 5 μm (A); 1 μm (B and C).