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. 2013 Jul;183(1):296–303. doi: 10.1016/j.ajpath.2013.03.020

Figure 3.

Figure 3

FAM190A changes during cell cycle. A: HeLa cells were treated with nocodazole for 16 hours or demecolcine for 24 hours. After microtubule depolymerizing drugs, FAM190A migrated slower. IB with mAb7 and anti–α-actin. B and C: HeLa cells were treated with nocodazole for 16 hours. At different times after drug washout, cells were prepared for IF. FAM190A foci were counted at different times. 0 foci, cells at interphase; 2 foci, cells in mitosis; >2 foci, cells under nocodazole effect or ongoing redistribution of FAM190A after drug release. n = foci counted from total number of cells at a time point. D: HeLa cells were synchronized at prometaphase by nocodazole preceded by thymidine block. Cells were harvested at different points after release from nocodazole and examined by IB. IB with mAb170, anti–cyclin E, anti–securin B, and anti-GAPDH. TN, thymidine-nocodazole block. E: HeLa cells were synchronized at G1/S-phase by double thymidine block. Cells were harvested at different times after release from block and examined by IB. IB with mAb170, anti–cyclin E, anti–securin B, and anti-GAPDH. DT, double thymidine block. Scale bar = 5 μm (B and C).