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. 2013 Jul;183(1):296–303. doi: 10.1016/j.ajpath.2013.03.020

Figure 5.

Figure 5

FAM190A is phosphorylated in mitosis. A: HeLa cells were incubated with MG132 for 4 hours and 6 hours and harvested in lysis solution. IB with mAb7 and anti–α-tubulin. B: HeLa cells were synchronized at prometaphase by nocodazole or released into MG132 after nocodazole block for 4 hours. FAM190A was captured with mAb170 and mAb62 and incubated with or without CIP for 60 minutes at 37°C. IB using mAb170. C: HeLa cells were synchronized to a different phase of cell cycle by double thymidine block (G1/S-phase), 2-hour release after double thymidine block (S-phase), or thymidine-nocodazole block (M-phase). Lysates were treated with CIP for 60 minutes. IB with mAb170 and mAb62 and α-tubulin. Un, unsynchronized cells. The arrowhead indicates a nonspecific band.