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. Author manuscript; available in PMC: 2013 Jul 6.
Published in final edited form as: Sci Transl Med. 2012 Apr 25;4(139):139ra84. doi: 10.1126/scitranslmed.3003923

Fig. 1.

Fig. 1

Huh7 RAD001 and BEZ235 synergistically inhibit mTOR signaling and decrease proliferation in Huh7 cells. Cells were treated with the indicated concentrations of RAD001 and/or BEZ235 for 48 hours. (A and B) Amounts of phosphorylated and total proteins were measured in cell lysates by Western blot analysis. Actin was used as a loading control. (C) Cell proliferation was measured using the CellTiter-Glo luminescent assay. The percent change in cell number from the beginning of the treatment relative to vehicle is plotted. Results are means ± SD (n = 3). ***P < 0.0001.