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Protocol of ovalbumin sensitization/challenge and Formula-3 treatment. Rats were sensitized with ovalbumin (OVA) (1 mg/mL × 1 mL daily per rat) intragastrically for 48 d. For the polydatin (PD) treatment group, the rats were orally treated with PD (30 mg/mL × 1 mL daily per rat) from days 49 to 52 after OVA sensitization. All the rat groups were challenged by 100 mg/mL OVA for 24 h at the end of day 52 and then sacrificed. PBS: Phosphate-buffered saline.
