Figure 5.

Polydatin therapy reduced mast cell degranulation and activation. A: Phosphate-buffered saline (PBS) group; B: Ovalbumin (OVA) group; C: OVA + polydatin (PD) group; D: PD group; E: Degranulated mast cells were counted from at least 500 total cells and the percentage of degranulation was calculated as degranulated cells against total cells; F: The release of histamine in serum (left panel), rat small intestine tissue, or peritoneal lavage solution (right panel) was measured by enzyme-linked immunosorbent assay. n = 8, ^aP < 0.05, ^bP < 0.01 vs PBS group; ^cP < 0.05, ^dP < 0.01 vs OVA group. The mast cells in rat small intestine tissue or peritoneal lavage solution were identified by toluidine blue stain. Mast cells were considered degranulated if at least 5 granules appeared outside the cell body. Arrows indicate degranulated mast cells (magnification, × 250).