Figure 4.
Reassessment of the effect of overexpression of wild-type and S97A-mutant Syt12 on spontaneous mini release in cultured cortical neurons. A, B, Representative traces (left) and summary graphs of the frequency and amplitudes (right) of mEPSCs (A) and mIPSCs (B) measured as a function of the addition of forskolin (50 μm). Neurons infected with a control lentivirus and lentiviruses overexpressing wild-type Syt12 (Syt12WT) or S97A-mutant Syt12 (Syt12S97A) were analyzed. Data shown are means ±SEM; numbers of cells/independent cultures analyzed are shown in the bars. Statistical assessments were performed by Student's t test (*p < 0.05; **p < 0.01; ***p < 0.001).