Table 2.
Effect of benzene fraction of Murraya koenigii on the mutagenicity induced by sodium azide (NaN3) using Salmonella typhimurium strains.
| Treatment |
Dose (µg/plate) | Number of His+ revertants colonies/plate | |||
|---|---|---|---|---|---|
| TA97a | TA98 | TA100 | TA102 | ||
| Spontaneous | 140.0 ± 4.0 | 31.0 ± 3.6 | 128.7 ± 9.8 | 245.7 ± 23.3 | |
| Positive control (NaN3) | 1.5 | 246.3 ± 16.0 | 52.7 ± 2.1 | 352.0 ± 33.0 | 343.3 ± 33.3 |
| a Murraya koenigii | 12.5 | 164.3 ± 12.3 | 46.0 ± 4.0 | 191.7 ± 13.3 | 286.7 ± 26.3 |
| 25 | 145.0 ± 15.5 | 37.3 ± 7.0 | 172.3 ± 18.9 | 260.0 ± 24.1 | |
| 50 | 124.7 ± 9.1 | 30.7 ± 8.5 | 153.0 ± 19.7 | 245.3 ± 24.4 | |
| 100 | 118.0 ± 10.5 | 24.0 ± 4.6 | 127.0 ± 15.5 | 228.3 ± 19.6 | |
| b Murraya koenigii + NaN3 | 12.5 | 225.3 ± 33.6 (25.6) | 51.3 ± 6.0 (20.0) | 316.3 ± 32.0 (22.3) | 332.3 ± 34.9 (19.4) |
| 25 | 200.7 ± 22.7* (45.1) | 45.3 ± 4.2 (47.8) | 258.0 ± 29.6* (52.3) | 313.0 ± 32.1 (36.4) | |
| 50 | 168.0 ± 22.3** (64.4) | 39.0 ± 8.2* (62.1) | 212.7 ± 26.6** (70.0) | 292.7 ± 18.6 (51.7) | |
| 100 | 137.3 ± 12.7*** (84.9) | 31.7 ± 6.5** (73.2) | 162.0 ± 21.0** (84.4) | 260.3 ± 14.0* (72.2) | |
|
| |||||
| F value | 18.069 | 8.288 | 33.690 | 6.917 | |
|
| |||||
| R 2 value | 0.999 | 0.953 | 0.969 | 0.996 | |
The data represented in the table is the mean ± SD values of three replicates. Mean values followed by asterisk are significantly different when compared to positive control at *P < 0.05, **P < 0.005, and ***P < 0.001. The values in parentheses are the inhibition rates of mutagenicity (%). Positive control: NaN3, sodium azide. aNegative control; bpreincubation test; R 2: linear regression analysis.