Table 3.
Effect of benzene fraction of Murraya koenigii on the mutagenicity induced by methyl methanesulfonate (MMS) using Salmonella typhimurium strains.
| Treatment |
Dose (µg/plate) | Number of His+ revertants colonies/plate | |||
|---|---|---|---|---|---|
| TA97a | TA98 | TA100 | TA102 | ||
| Spontaneous | 140.0 ± 4.0 | 31.0 ± 3.6 | 128.7 ± 9.8 | 245.7 ± 23.3 | |
| Positive control (MMS) | 1 | 443.7 ± 19.9 | 51.0 ± 2.7 | 952.7 ± 46.0 | 1195.3 ± 79.0 |
| a Murraya koenigii | 12.5 | 164.3 ± 12.3 | 46.0 ± 4.0 | 191.7 ± 13.3 | 286.7 ± 26.3 |
| 25 | 145.0 ± 15.5 | 37.3 ± 7.0 | 172.3 ± 18.9 | 260.0 ± 24.1 | |
| 50 | 124.7 ± 9.1 | 30.7 ± 8.5 | 153.0 ± 19.7 | 245.3 ± 24.4 | |
| 100 | 118.0 ± 10.5 | 24.0 ± 4.6 | 127.0 ± 15.5 | 228.3 ± 19.6 | |
|
b
Mu
rr
ay
a
koenigii+ MMS |
12.5 | 388.3 ± 31.2 (19.8) | 50.3 ± 4.0 (13.4) | 802.3 ± 57.1* (19.8) | 862.3 ± 58.8** (36.6) |
| 25 | 309.0 ± 30.8* (45.1) | 45.0 ± 4.6 (43.9) | 565.0 ± 45.1*** (49.7) | 675.3 ± 56.9*** (55.6) | |
| 50 | 239.7 ± 20.2** (63.9) | 38.3 ± 4.0* (62.3) | 440.7 ± 48.6*** (64.0) | 518.0 ± 32.1*** (71.3) | |
| 100 | 183.0 ± 17.5*** (80.0) | 31.0 ± 5.6** (74.1) | 262.3 ± 31.7*** (83.6) | 363.7 ± 37.1*** (86.0) | |
|
| |||||
| F value treatment | 81.635 | 9.877 | 225.881 | 178.255 | |
|
| |||||
| R 2 value | 0.989 | 0.958 | 0.978 | 0.996 | |
The data represented in the table is the mean ± SD values of three replicates. Mean values followed by asterisk are significantly different when compared to positive control at *P < 0.05; **P < 0.005 and ***P < 0.001. The values in parentheses are the inhibition rates of mutagenicity (%). Positive control: MMS: methyl methanesulfonate. aNegative control; bPre-incubation; R 2: Linear regression analysis.