Table 4.
Effect of benzene fraction of Murraya koenigii on the mutagenicity induced by benzo(a)pyrene with metabolic activation using Salmonella typhimurium strains.
| Treatment | Dose (µg/plate) | Number of His+ revertants colonies/plate | |||
|---|---|---|---|---|---|
| TA97a | TA98 | TA100 | TA102 | ||
| Spontaneous | 143.7 ± 13.2 | 37.0 ± 3.0 | 135.3 ± 9.5 | 316.0 ± 14.5 | |
| Positive control (BP) | 1 | 720.3 ± 28.5 | 155.0 ± 7.6 | 695.7 ± 17.0 | 648.0 ± 17.1 |
| a Mu rr ay a koenigii | 12.5 | 142.0 ± 18.3 | 50.3 ± 2.5 | 132.7 ± 16.0 | 265.0 ± 28.2 |
| 25 | 156.0 ± 14.4 | 42.7 ± 3.5 | 155.3 ± 17.2 | 296.3 ± 17.6 | |
| 50 | 168.7 ± 21.7 | 35.0 ± 3.6 | 168.0 ± 18.0 | 322.7 ± 31.1 | |
| 100 | 181.3 ± 27.1 | 28.0 ± 3.6 | 175.3 ± 20.6 | 340.3 ± 31.3 | |
| b Mu rr ay a koenigii+ BP | 12.5 | 592.3 ± 44.5* (22.1) | 132.7 ± 19.2 (21.3) | 540.0 ± 50.1** (27.7) | 560.3 ± 31.2* (22.9) |
| 25 | 501.7 ± 36.6** (38.7) | 101.0 ± 19.3* (48.1) | 435.3 ± 46.1*** (48.2) | 501.7 ± 16.9*** (41.9) | |
| 50 | 382.7 ± 35.6*** (61.2) | 80.7 ± 12.7*** (61.9) | 352.3 ± 30.7*** (65.1) | 436.0 ± 38.2*** (65.2) | |
| 100 | 276.0 ± 26.5*** (82.4) | 53.3 ± 13.9*** (80.1) | 253.7 ± 21.4*** (84.9) | 382.0 ± 29.2*** (86.0) | |
|
| |||||
| F value treatment | 168.149 | 49.036 | 149.651 | 67.488 | |
|
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| R 2 value | 0.996 | 0.982 | 0.999 | 0.999 | |
The data represented in the table is the mean ± SD values of three replicates. Mean values followed by asterisk are significantly different when compared to positive control at *P < 0.05; **P < 0.005 and ***P < 0.001. The values in parentheses are the inhibition rates of mutagenicity (%). Positive control: BP: benzo(a)pyrene.aNegative control; bPre-incubation test; R 2: Linear regression analysis.