Table 5.
Effect of benzene fraction of Murraya koenigii on the mutagenicity induced by 2-aminofluorene (2-AF) with metabolic activation using Salmonella typhimurium strains.
| Treatment | Dose (µg/plate) | Number of His+ revertants colonies/plate | |||
|---|---|---|---|---|---|
| TA97a | TA98 | TA100 | TA102 | ||
| Spontaneous | 143.7 ± 13.2 | 37.0 ± 3.0 | 135.3 ± 9.5 | 316.0 ± 14.5 | |
| Positive control (2AF) | 5 | 328.0 ± 12.0 | 262.3 ± 17.2 | 502.0 ± 19.1 | 1430.3 ± 32.5 |
| a Murraya koenigii | 12.5 | 142.0 ± 18.3 | 50.3 ± 2.5 | 132.7 ± 16.0 | 265.0 ± 28.2 |
| 25 | 156.0 ± 14.4 | 42.7 ± 3.5 | 155.3 ± 17.2 | 296.3 ± 17.6 | |
| 50 | 168.7 ± 21.7 | 35.0 ± 3.6 | 168.0 ± 18.0 | 322.7 ± 31.1 | |
| 100 | 181.3 ± 27.1 | 28.0 ± 3.6 | 175.3 ± 20.6 | 340.3 ± 31.3 | |
| b Murraya koenigii+ 2-AF | 12.5 | 276.3 ± 31.1 (27.8) | 200.7 ± 17.0* (29.1) | 422.3 ± 32.7* (21.6) | 1185.3 ± 77.0** (21.0) |
| 25 | 248.7 ± 28.0* (46.1) | 161.0 ± 12.5** (46.1) | 346.3 ± 21.6*** (44.9) | 960.7 ± 70.8*** (41.4) | |
| 50 | 220.0 ± 25.0** (67.8) | 114.7 ± 13.1*** (65.0) | 280.7 ± 27.0*** (66.3) | 655.0 ± 55.3*** (70.0) | |
| 100 | 202.3 ± 14.2*** (85.7) | 70.0 ± 7.6*** (82.1) | 205.0 ± 18.0*** (90.9) | 462.0 ± 32.9*** (88.8) | |
|
| |||||
| F value treatment | 24.643 | 197.239 | 37.820 | 276.179 | |
|
| |||||
| R 2 value | 0.987 | 0.999 | 0.999 | 0.994 | |
The data represented in the table is the mean ± SD values of three replicates. Mean values followed by asterisk are significantly different when compared to positive control at *P < 0.05; **P < 0.005 and ***P < 0.001. The values in parentheses are the inhibition rates of mutagenicity (%). Positive control: 2-AF, 2-aminoflourene.aNegative control; bPre-incubation test; R 2: Linear regression analysis.