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Probing TgADF-Actin interactions by ITC. The negative peaks indicate an exothermic reaction. The area under each peak represents the heat released after an injection of TgADF into ADP-G-Actin solution. (Lower) Binding isotherms obtained by plotting peak areas against the molar ratio of TgADF to ADP-G-Actin. The lines represent the best-fit curves obtained from least-squares regression analyses assuming a one-site binding model.
