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. Author manuscript; available in PMC: 2013 Jul 7.
Published in final edited form as: Pharm Res. 2011 Apr 13;28(8):2034–2044. doi: 10.1007/s11095-011-0429-2

Figure 2.

Figure 2

Induction of CYP2B6 and CYP3A4 by CPA and IFO in human primary hepatocytes. Human hepatocytes from liver donors (HL-19 and HL-25) were treated with CITCO (1μM), RIF (10μM) or CPA and IFO at indicated concentrations for 24 hr (mRNA) and 72 hr (protein), respectively. The expression of mRNA for CYP2B6 (A, C) and CYP3A4 (B, D) was analyzed using RT-PCR; and related protein expression was detected using immunoblotting analysis as outlined in “Materials and Methods”. RT-PCR data are expressed as mean ± SD (n=3). **: p < 0.01. Densitometry of CYP2B6 and CYP3A4 proteins was normalized against β-actin expression.