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Inhibition of PS-coumarin probe 4 translocation by ATP depletion. Cells were incubated with 50 mM 2′-deoxyglucose and 0.1% sodium azide in glucose free media for 30 min at 37 °C then treated with 10 μM of 4. After incubation at 37 °C for 30 min, cells were washed and imaged at 60X.
