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Inhibition of PS-coumarin probe 4 translocation by covalent sulfhydryl modification. Cells were incubated with 10 mM of iodoacetamide in media at 37 °C for 30 min, then treated with 10 μM of 4. After incubation at 37 °C for 30 min, cells were washed and imaged at 60X.
