Figure 5.
Representative Western blots (A) and quantitative analysis of pAkt (B) expression in groups of sham operation (sham), HI or treated with limb ischemic postconditoning (HI+PostC), naloxone+postconditioning (naloxone+HI+PostC), and morphine (HI+morphine); n=6 in each group at 48 hours after HI. The pAkt expression remained decreased in the HI group at 48 hours after HI. Limb ischemic postconditioning and morphine treatment restored the Akt activity. Naloxone blocked this postconditioning-mediated effect. Values are the mean±SEM; *P<0.05 versus HI group; #P<0.05 versus sham-operated group.