Figure 5.
Confocal immunofluorescent labeling of Nav1.4 (red). Panel A, Positive control: a skeletal muscle section immunostained with Nav1.4 antibody. Panel B, Negative control: a left ventricular section without Nav1.4 injection, immunostained with Nav1.4 antibody. Panel C. Representative images of left ventricular sections at the injection site immunostained with Nav1.4 antibody. The antibody stained intensely at the longitudinal end of the cardiomyocytes in the Nav1.4 injected sections. Panel D, enlargement of section in panel C. DAPI (blue) was used as a nuclear stain. Scale bars are 20 microns in Panels A–C and 5 microns in Panel D.