Figure 6.

Increased ethanol intake by UChB rats chronically exposed to ethanol, subsequently deprived and ethanol re-exposed. Marked inhibition of intake following the administration of an anticatalase lentiviral vector. Animals with ad-libitum access to 10 and 20% ethanol and water for 60 days. Baseline data correspond to the average of ethanol intake restricted to only 1 h a day, for 7 days immediately prior to alcohol deprivation. A single intra-ventral tegmental area injection of an anticatalase-lentiviral vector inhibited the first 1-h ethanol intake after the first and second ethanol deprivation (ADE) periods of 15 days. The total height of each bar represents the sum of ethanol intake (g ethanol/kg/60 min) of the 10% solution (empty bars) plus that of the 20% solution (gray bars). The -/ /- symbol in the x-axis represents the 15-day deprivation period. (A) Control viral vector. (B) Anticatalase viral vector. Arrows indicate the administration of either control lentiviral vector or anticatalase-lentiviral vector prior to the 15 days of deprivation. The first and second re-exposure consumptions were significantly different from baseline. The inhibition induced by anticatalase vector administration was 67% (p < 0.001) after the first deprivation period, and 80% (p < 0.001) after the second deprivation period. Note also the marked increases in ethanol binge-drinking induced in control vector treated animals after the first and second ethanol intake and deprivation cycles (From Tampier et al., 2013).