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. 2013 Jul;183(1):108–118. doi: 10.1016/j.ajpath.2013.04.004

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© 2013 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

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Enamel matrix proteins in rat mandibular incisors on day 30. Enamel matrix proteins were sequentially extracted from secretory-stage enamel on day 30 and analyzed by Western blotting with specific antibodies for enamelin (A), amelogenin (B), and ameloblastin (C). D: Densitometric quantification revealed a significant increase (2.12-fold ± 0.02) of enamelin adsorbed to the enamel crystals (PBS fractions) in BPA-treated rats (P = 0.02). All data are means ± SEM (n = 3). ^∗P < 0.05. IHC analysis of enamelin (E), amelogenin, (F), ameloblastin (G), and serum albumin (H). Scale bars: 70 μm (G); 200 μm (H). En, enamel; Am, ameloblasts.