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. 2013 Jul;183(1):119–130. doi: 10.1016/j.ajpath.2013.03.025

Figure 2.

Figure 2

Ang II reduces nephrin expression through the involvement of Notch1 in cultured human podocytes. A: Representative images of nephrin staining in human podocytes exposed for 3 hours to 100 nmol/L Ang II. B: Nephrin mRNA expression in human podocytes exposed to Ang II alone or in combination with 1 μmol/L γ-secretase inhibitor, GSI X. ∗P < 0.01 versus control podocytes; †P < 0.01 versus Ang II alone. C, top panel: Representative images of ICN1 staining (red) and cell nuclei (DAPI, blue). Bottom panel: Quantification of the percentage of nuclei positive for ICN1 at different time intervals. Transforming growth factor (TGF)-β (5 ng/mL for 120 minutes) was used as a positive control. ∗∗P < 0.001 versus control podocytes.