Figure 3.

Role of the Notch1 pathway on Snail activation in Ang II–treated podocytes. A: Snail protein expression in podocytes exposed to Ang II in the presence or absence of 1 μmol/L γ-secretase inhibitor, GSI X, at different time intervals. Representative images of Snail staining (red) and nuclei (DAPI, blue) and quantification of Snail-positive nuclei (%). ^∗P < 0.001 versus control; ^†P < 0.001 versus Ang II. B: Snail mRNA expression in podocytes exposed to Ang II for 1 hour in the presence or absence of GSI X and in podocytes knocked down for HES1 with siHES1 or control nontarget siRNA (siNULL). ^∗P < 0.001 versus control; ^†P < 0.001 versus Ang II or Ang II + siNULL. C: Representative images of Snail staining (red with DAPI in blue) in siNULL- or siHES1-transfected podocytes exposed to Ang II at different time intervals and relative quantification of Snail-positive nuclei (%). ^∗P < 0.001 versus control; ^†P < 0.001 versus Ang II + siNULL.