Figure 4.

Ramipril reduced proteinuria, improved renal histological features, and restored nephrin and Snail expression in ZDF rats. A: Urinary protein excretion rate in ZDF rats receiving vehicle or ACE inhibitor and controls measured at 4 (pretreatment) and 8 months. ^∗P < 0.01 versus controls; ^†P < 0.01 versus untreated. B: Representative images of PAS reagent–stained kidney sections of control and diabetic animals that received ramipril or did not receive ramipril and were sacrificed at 8 months. C: Photomicrographs of nephrin (red) and Snail (green) immunofluorescence on frozen kidney sections. Nuclei were stained with DAPI.