Figure 7.

Ramipril restores Notch1 expression in diabetic patients. Representative images of Notch1 glomerular expression assessed by ICN1 immunoperoxidase. Insets, twofold enlargement of boxed area. Arrows, mild and cytoplasmic signal in controls that increases and becomes nuclear in diabetic glomeruli. The staining is negative in the absence of primary antibody (minus). Double immunofluorescence of ICN1 (green) and WT1 (red) reveals Notch1 activation in podocytes of untreated diabetic patients. Arrows, colocalization of the two signals (yellow). The histogram shows the percentage of ICN1-positive nuclei. ^∗P < 0.05 versus control group; ^†P < 0.01 versus diabetic patients.