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The wavelength of flavonoid absorbance is 280 nm. The injection volume was 10 µL. Ethanol stock solution containing (1) rutin, (2) narirutin, (3) naringin, (4) hesperidin, (5) neohesperidin, (6) neohesperidin dihydrochalcone (NHDC), (7) naringenin, (8) hesperetin, (9) aprgrnin, (10) nobiletin, (11) tangeretin, (12) auraptene was diluted with ethanol to obtain a series concentration of working solutions, and was subjected to HPLC analysis.
