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. 2013 Jul 8;3:178. doi: 10.3389/fonc.2013.00178

Figure 1.

Figure 1

(A) Southern blot analysis of telomere length in HT1080 parental (S) or telomerase-overexpressing (L) cells and in HeLa parental (S) or a subclone with long telomeres (L) (2). MW, molecular weight. (B) Northern blot analysis of TERRA and ACTB (control) in the four cell lines described in (A), with or without alkaline treatment of the agarose gel prior to transfer. *High molecular weight molecules that only transfer after alkaline treatment. (C) Quantification from (B). (D) Cartoon representing human chromosome ends and subtelomeric primers (arrowheads) that are used to quantify TERRA molecules from various chromosome ends by qRT-PCR. (E) qRT-PCR analyses of TERRA molecules transcribed from either 1q/2q/10q/13q or 15q in the four cell lines described in (A). Values were normalized to parental cell lines and error bars represent SD. All methods used here have been described in Arnoult et al. (2).