Skip to main content
. Author manuscript; available in PMC: 2013 Jul 8.
Published in final edited form as: Proteomics Clin Appl. 2012 Aug;6(0):346–350. doi: 10.1002/prca.201200014

Figure 1. Lectin-based detection of glycans in microarray formats.

Figure 1

The types of microarrays depicted are lectin arrays, antibody-lectin sandwich arrays, and glycoprotein arrays. A detection strategy using a fluorescent dye is depicted, although other detection methods could be used, such as surface-plasmon resonance or chemiluminescence. Antibody-lectin sandwich arrays (middle row) enable the detection of glycans on specific proteins captured from biological solutions. Multiple antibodies are immobilized on a planar support, and the captured proteins are probed using biotinylated detection antibodies, followed by fluorescence detection using phycoerythrin-labeled streptavidin.