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. 2013 Jul 8;3:2157. doi: 10.1038/srep02157

Figure 4. Dose-dependent increase in GCH1 transgene expression.

Figure 4

Note that the antibody used here does not cross react with the rodent protein and thus only shows the transgenic human GCH1 expressed from the AAV vector. Intrastriatal injections of 9E8 gc TH-GCH1 vector (B) resulted in very few GCH1 positive neurons in the striatum and globus pallidus, whereas the transduction efficiency increased with vector doses of 5E9 gc (C), 1E10 (D) and 1E11 gc (E). The two high doses resulted in complete coverage of striatum and globus pallidus (D,E). The transduction spread was similar in the GCH1-GCH1 control vector group (E,F). ctx = cortex, cc = corpus callosum, gc = genomic copies, gp = globus pallidus, lv = lateral ventricle, str = striatum. Scale bar in a represent 0.2 mm in all panels.