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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cell Signal. 2013 May 22;25(9):1754–1761. doi: 10.1016/j.cellsig.2013.05.010

Fig. 2.

Fig. 2

Role of HER2 in Brk protein stability. (A) SUM190 cells were transfected with a HER2-specific siRNA#1 or control siRNA for 48 h. The cells were then exposed to 10 μM cycloheximide (CHX) in 0.5% FBS medium for the indicated times. The levels of HER2 and Brk were detected by Western blotting with the indicated antibodies. β-actin was used as a loading control. Levels of HER2 and Brk relative to the internal control β-actin were quantified and plotted as shown. (B) MCF7-neo and MCF7-HER2 cells were exposed to CHX (10 μM) in 0.5% FBS medium for the indicated times. The levels of HER2 and Brk were detected by Western blotting with the indicated antibodies. β-actin was used as a loading control. Levels of HER2 and Brk relative to the internal control β-actin were quantified and plotted as shown.