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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cell Signal. 2013 May 22;25(9):1754–1761. doi: 10.1016/j.cellsig.2013.05.010

Fig. 4.

Fig. 4

Regulation of calpastatin level by HER2. (A) SUM190 cells were transduced with three different HER2 lentiviral shRNAs respectively or control lentiviral shRNA for 48 h and then cultured in selecting medium with 2 μg/mL puromycin for 1 week. The cells were lysed for Western blotting with the indicated antibodies (top), and for calpain activity assay (bottom). (B) Pairs of control siRNA-treated or HER2 siRNA-treated SUM190 cells and MCF7-neo and MCF7-HER2 cells were lysed for Western blotting with the indicated antibodies.