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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cell Signal. 2013 May 22;25(9):1754–1761. doi: 10.1016/j.cellsig.2013.05.010

Fig. 5.

Fig. 5

Upregulation of calpastatin by HER2 in a MEK/Erk-dependent manner. (A and B). MCF7-neo and MCF7-HER2 cells were treated with the indicated doses of LY294002 (A) or PD98059 (B) in 0.5% FBS DMEM for 24 h. Cell lysates were prepared and analyzed by Western blotting with the indicated antibodies and by calpain activity assay (bottom). (C) SUM190 cells were treated with PD98059 in 0.5% FBS DMEM for 24 h. Cell lysates were prepared and analyzed by Western blotting with the indicated antibodies and by calpain activity assay (bottom). (D) SUM190 cells were transfected with control siRNA or HER2 siRNA for 24 h and then transfected with constitutively active MEK1 or control vector as shown for an additional 24 h. Cells were lysed for Western blotting with the indicated antibodies.