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The sequences, Tm and size of amplicons of the designed primers used in PCR for analysis of HSV-1/Vero cell DNA.
| Primers | Target genes |
Nucleotide sequence (5’ to 3’) | Tm (°C) |
Amplicon (nt) |
|---|---|---|---|---|
| gD1f | HSV-1 US6 | AGACGTCCGGAAACAACCCTACAA | 64.6 | 752 |
| gD1r | ACACAATTCCGCAAATGACCAGGG | 64.6 | ||
| GFPf | HSV-1 GFP | TGACCCTGAAGTTCATCTGCACCA | 64.6 | 717 |
| GFPr | AACTCCAGCAGGACCATGTGAT | 62.7 | ||
| VP11/12f | HSV-1 US46 | ACCAAGCCTTGATGCTCAACTCCA | 64.6 | 957 |
| VP11/12r | ACAACACGGTTCCCGAGAGTTTGA | 64.6 |
