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. Author manuscript; available in PMC: 2013 Jul 8.
Published in final edited form as: Am J Reprod Immunol. 2011 Aug 7;67(1):17–27. doi: 10.1111/j.1600-0897.2011.01056.x

Figure 5. Swan-71 cells specifically recruit iTregs and this process is increased in the presence of an infection.

Figure 5

(A) Trophoblast cells were cultured in a 24-well flat bottom polystyrene plates in complete DMEM 10% FCS. Upon reaching 70% confluency, cells were treated in the absence or presence of LPS, PGN, or Poly[I:C] representing a bacterial or viral infection. Then, 8µm inserts were added containing the differentiated naïve T cells labeled with PKH26. After 48 h of culture, cells from the lower compartment were recovered and analyzed by FACS. The spontaneous migration was considered as the % of PKH26+Foxp3+ cells recovered from the lower compartment without attraction-stimuli (*p<0.05 Student T-test). (B) Figure shows representative dot plots from one of three experiments and shows the frequency of the migrated CD4+Foxp3+ population expressed as a percentage.