Skip to main content
. Author manuscript; available in PMC: 2013 Jul 8.
Published in final edited form as: Oncogene. 2012 Feb 20;32(1):39–49. doi: 10.1038/onc.2012.33

Figure 2. Spheroid formation is an EMT process.

Figure 2

Figure 2

Figure 2

(A EMT array showed that the transition from CD44+/MyD88+ EOC stem cells to MSFCs is characterized by upregulation of genes associated with EMT. Array was done using two clones for each stage of differentiation;

(B Down-regulation of epithelial genes (CK7 and CK19) and up-regulation of mesenchymal genes (FOXC2, TWIST1, TGFB3, SLUG, VIMENTIN) during spheroid formation were validated by Real-time PCR;

(C TGFβ secretion was significantly increased after spheroid formation; EOCSC - EOC stem cells; MSFC - mesenchymal spheroids forming cells. . Each experiment was done in triplicate using three different cell clones.