Fig. 2.

Neutrophil gene expression is altered during F. tularensis infection. PMNs were left untreated or infected with LVS for 0- 48 h and neutrophil gene expression was analyzed using Affymetrix human oligonucleotide microarrays. a PCA depicts variation and clusters within the data set in three dimensions. The x-axis (PC 1) indicates the largest amount of variation (28.1%), the y-axis (PC 2) the second largest amount of variation (9.9%) and the z-axis (PC 3) shows all of the remaining variation (7.3%), for 45.3% variation in total. b Differentially expressed genes were categorized as up- or downregulated and enumerated at the indicated time points. c Confirmation of the microarray data. Differential expression of the indicated genes was quantified using real-time Q-PCR. Data shown are the mean ± SD from experiments performed using cells from three different donors. Inset Graph shows the strong correlation (Spearman correlation coefficient r = 0.9136 and p = 0.0046) between the Q-PCR and microarray data.