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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Nat Neurosci. 2013 Jun 9;16(7):874–883. doi: 10.1038/nn.3429

Figure 8. Arc localization to the nucleus mediates homeostatic plasticity.

Figure 8

(a) Representative images of surface GluA1 staining of neurons transfected with a control plasmid, Arc-mNES or Arc-NES and treated with bicuculline for 48 h.

(b) Quantification of surface GluA1 staining in neurons transfected with a control plasmid, Arc-mNES or Arc-NES shows Arc localization to the nucleus prevents downscaling. N=65 for control, N=64 for bicuculline, N=37 for Arc-mNES, N=50 for Arc-mNES+bicuculline, N=50 for Arc-NES, N=50 for Arc-NES+bicuculline from four independent cultures.

(c) Quantification of Arc levels in untransfected neurons using Arc staining and neurons transfected with ABCDEFG-Arc-His-Flag-HI using Flag staining, with and without 8-h BDNF treatment. N=55 for endogenous Arc, N=37 for endogenous Arc + BDNF, N=40 for ABCDEFG-Arc-His-Flag-HI, N=37 for ABCDEFG-Arc-His-Flag-HI+BDNF from three independent cultures.

(d) Representative images of ABCDEFG-Arc-His-Flag-HI construct expression with and without BDNF treatment in neurons stained with anti-Flag antibody.

(e) Representative images of surface GluA1 staining of Arc−/− neurons transfected with a control plasmid, ABCDEFG-Arc-mNES-HI or ABCDEFG-Arc-NES-HI and treated with bicuculline for 48 h.

(f) Quantification of surface GluA1 staining in neurons transfected with a control plasmid, ABCDEFG-Arc-mNES or ABCDEFG-Arc-NES shows Arc localization to the nucleus rescues downscaling in Arc knockout neurons. N=58 for control, N=47 for control+bicuculline, N=59 for ABCDEFG-Arc-mNES-HI, N=45 for ABCDEFG-Arc-mNES-HI+bicuculline, N=40 for ABCDEFG-Arc-NES-HI, N=30 for ABCDEFG-Arc-NES-HI+bicuculline from at least three independent cultures.

***, p<0.001, n.s., not significant, by ANOVA. Error bars show 95% confidence intervals. Scale bars=10 μm.