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LSIV-IMS-MS (A) total mass spectra and (B) two-dimensional plot of drift time vs. m/z of delipified mouse brain tissue (I) spotted and (II) spray coated with 100% 2-NPG matrix. Insets show (A) isotopic distributions of +2 charge state ion of the identified peptide MBP fragment, (I.B) drift times of charge state +4 and +1 ions, and (II.B) isotopic distribution of the +2 ions. Insets of higher charge states in (I) are included in Figure S5.
