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. 2013 Jun 18;2013:509502. doi: 10.1155/2013/509502

Figure 5.

Figure 5

Effects of GW0742 treatment on AMPK/ACC phosphorylation and CPT-1 expression in the gastrocnemius of mice fed with HFCS diet. Total AMPK protein expression and Thr172 phosphorylation (a), total ACC protein expression and Ser79 phosphorylation (b), and CPT-1 expression (c) were analyzed by western blot on the gastrocnemius homogenates obtained from mice fed with a standard (control) or HFCS diet (HFCS) for 30 weeks and treated with GW0742 (1 mg/kg/day) added during the last 16 weeks (control + GW; HFCS + GW). Densitometric analysis of the bands is expressed as relative optical density (O.D.), corrected for the corresponding tubulin contents, and normalized using the related control band. The data are means ± SD of three separate experiments. *P < 0.01 versus HFCS.