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IVIG induced Syk tyrosine phosphorylation in BMDC stimulated with LPS. Cells were stimulated with LPS (500 ng/mL) in the presence or absence of IVIG (10 mg/mL) for 15 min. Cell lysates were incubated with the anti-Syk antibody and precipitated by Protein A/G. Immunoprecipitates were analyzed by immunoblotting with HRP-conjugated, anti-phosphotyrosine antibody (4G10) or the anti-Syk antibody.
